Fitness, stability, and conformational entropy of single amino acid mutations.

Below wildtype, the mutations are ordered by descending fitness.
aSubstitutions come from Rokyta et al. [14] and Miller et al. [15].
bFitness is defined as population doublings per hour.
cThermal decay rate is a proportion of the ancestral decay rate; values less than 1.0 are more stable than ancestor.
dΔΔG in units of kcal/mol is estimated protein-protein interaction via molecular dynamics simulation; values less than 0.0 indicate more stable than ancestor.
eConformational entropy, ΔS, in units of kcal/mol K is estimated via molecular dynamics simulation.
*Estimates are median values of individual data points.
**95% confidence intervals are based on +/- 3.2 absolute errors (see Appendix S1).
***95% confidence intervals based on +/- 2.5 absolute errors. The difference in number of absolute errors used reflects differences in sample size.