Direct Induction of Neural Progenitor Cells Transiently Passes Through a Partially-reprogrammed State
收藏NIAID Data Ecosystem2026-03-10 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP068455
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资源简介：
During giNPC induction process, cell samples were harvested at various induction days including named Day1, Day4, Day7, Day10, Day14, Day17 and Day21. Meanwhile, samples from primary neurosphere-like cluster (named pre-NPCs), established giNPC cell line at passage 6 (named giNPCs P6), Brain-NPCs as well as Teratoma were also collected. Total RNA was isolated from cell pellets by Trizol reagent and RNA-Seq library were generated using KAPA Strandard mRNA-Seq Kits according to the manufacturer''s recommendations. Overall design: mRNA profiles of 11 cell types, generated by deep sequencing in duplicate, using HiSeq 2000. Paired-end 100-bp sequencing was applied.
创建时间：
2017-09-17
