George von Dassow, Koen J.C. Verbrugghe, Ann L. Miller, Jenny R. Sider, William M. Bement (2011) CIL:13399, Dendraster excentricus. CIL. Dataset

Microtubules in sand dollar embryo expressing three tandem GFPs fused to the ensconsin microtubule-binding domain (EMTB-3G; Faire et al., 1999). Eight-cell sand dollar embryo; single microtubules grow as far as the cell surface in all directions (equatorially in the 01:56 frame, tropically in the 01:08 frame, and toward the pole in the 03:32 frame). Astral microtubules reach the polar cortex most densely in anaphase (08:48) but also reach the equator before furrowing (frame 10:48). Embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14–17°C, which is tolerated well. Video corresponds to Fig 1C and third segment in Video 1 from J Cell Biol. 2009 Dec 14;187(6):831-45.

微管在沙钱胚胎中表达三种串联的绿色荧光蛋白（GFPs），与微管结合蛋白（EMTB-3G；Faire 等人，1999年）融合。八细胞沙钱胚胎中，单条微管向四面八方延伸至细胞表面（在01:56帧中为赤道方向，在01:08帧中为热带方向，在03:32帧中向极区方向）。星状微管在分裂中期（08:48）最密集地达到极区皮质，但在细胞皱缩之前也达到赤道（第10:48帧）。胚胎使用激光扫描共聚焦显微镜（Radiance 2000；Bio-Rad 实验室）成像，该显微镜安装在复合显微镜（E800；尼康）上，并使用40× 1.3 NA Plan-Fluor 油镜头。室温保持在14-17°C，这一温度范围得到胚胎的良好耐受。视频与图1C及视频1的第三部分相对应，见J Cell Biol. 2009年12月14日；187(6):831-45。