Identification of different miRNAs in Alzheimer's disease and vascular cognitive impairment and dementia

There are many subtypes of dementia without any minimally invasive, low-cost and efficient diagnostic biomarkers. MicroRNA (miRNA) are easily gained and accessible from patients, which may be a promising biomarker for dementia diagnosis and differential diagnosis. We make a completely expression analysis of serum miRNA from 24 participants including post-stroke cognitive impairment (PSCI) group (n=6), post-stroke non-cognitive impairment (PSCI) group, Alzheimer's Disease (AD) group (n=6) and normal control group (NC) group (n=6). Different expression miRNA (DE -miRNA) were screened by LIMMA ebayes method, Benjamini-Hochberg check and fold change (P < 0.05, |log2FC|≥1), we construct a DE-miRNA-key gene-signaling pathway network, the roles of DE-miRNA and related genes and signaling pathways in AD and PSCI were deduced through comprehensive network construction and literature search. Totally, plasma of 24 patients who have been admitted to The Department of Neurology at the First Hospital of Jilin University between 2021 and 2022 were selected. We conduct a comprehensive miRNA expression analysis of plasma samples from group1:post-stroke cognitive impairment (PSCI) group (n=6), group 2: post-stroke non-cognitive impairment (PSCI) group, group 3: Alzheimer's Disease (AD) group (n=6) and group 4: normal control group (NC) group (n=6).