Gene expression of primary Glioblastoma cell lines

We investigated the gene expression profiles of primary Human GBM cell lines. To represent the complexity of the GBM tissues, we uesd cell lines belonging to two different subtypes (HuTu10, proneural; HuTu13, Mesenchymal). Two primary Glioblastoma cell lines were transfected with control siRNAs. Each sample was in quadruplicate. Two days after transfection, cells were harvested for RNA extraction; total RNA was then used for Affymetrix Microarray profiling.