Genes responsible for the HPHB cycle in the identified genomes and their identity with those in SBR1093.

The identified genomes are downloaded from NCBI FTP with the accession names Acidianus hospitalis W1 uid66875 (Ac. hospitalis), Metallosphaera sedula DSM 5348 uid58717 (M. sedula), Sulfolobus tokodaii 7 uid57807 (S. tokodaii), Candidatus Nitrososphaera gargensis Ga9 2 uid176707 (N. gargensis), Afipia felis ATCC 53690 uid179396 (Af. felis), Bradyrhizobium STM 3843 uid80711 (B. sp. STM3843), Oligotropha carboxidovorans OM5 uid72795 (O. carboxidovorans). The enzymes in bold are bifunctional enzymes in the HPHB pathway, and columns filled with//indicate that there were no hit enzymes in the genome. M. sedula, S. tokodaii and Ac. hospitalis are members of Sulfolobales and have the potential for autotrophic metabolism via the reported HPHB pathway, whereas B. sp. STM3843, Af. felis and O. carboxidovorans are members from Bradyrhizobiaceae with facultative autotrophic metabolism via an unknown carbon fixation pathway. The accession number for Acidianus hospitalis should have ‘YP0044’ before the presented numbers, and Metallosphaera should have ‘YP0011’, Sulfolobus ‘NP3’, Nitrososphaera ‘YP0068’, Afipia ‘ZP114’, Bradyrhizobium ‘ZP0943’, and Oligotropha ‘YP0046’.Abbreviations for functional enzymes: Acryloyl-CoA reductase (ACR); Acetyl/propionyl-CoA carboxylase (ACC); 3-Hydroxypropionyl-CoA dehydratase (HPCD); Methylmalonyl-CoA epimerase (MCE); Malonic semialdehyde reductase (MSR); Methylmalonyl-CoA mutase (MCM); 4-Hydroxybutyrate-CoA ligase (HBCS); 3-Hydroxypropionate-CoA ligase (HPCS); Acetoacetyl-CoA β-ketothiolase (ACK); Succinyl/Malonyl-CoA reductase (MCR); Crotonyl-CoA hydratase (CCH); 4-Hydroxybutyryl-CoA dehydratase (HBCD); Succinic semialdehyde reductase (SSR).Genes responsible for the HPHB cycle in the identified genomes and their identity with those in SBR1093.