Long non-coding RNAs induced by TGF-beta in three breast (cancer) cell lines

Long non-coding RNAs (lncRNAs) are emerging as pivotal modulators of signaling trnasduction, and thereby regulate multiple pathological processes including cancer. TGF-beta signaling contributes to cancer metastasis by inducing epithelial-to-mesenchymal transition (EMT). To screen lncRNAs that are induced by TGF-beta, MCF10A-M1, MCF10A-M2 and MDA-MB-231 cells were stimulated with TGF-beta (5 ng/mL) fo 0 h, 2 h, 8 h and 24 h. RNA was extracted from those cells and analyzed by RNA-seq. MCF10A-M1, MCF10A-M2 and MDA-MB-231 cells in biological triplicates were stimulated with TGF-beta signaling inhibitor SB431542 (SB) or TGF-beta ligand fo 0 h, 2 h, 8 h and 24 h. In total, 36 samples were analyzed.