Transcriptomic analysis of Hela Ezrin Ko cells

Ezrin (EZR), a cell membrane to cytoskeleton linker protein, plays key roles in maintaining cellular homeostasis. Its deregulation at various levels strongly correlates with cancer progression, metastasis and higher patient mortality. However, the precise mechanistic basis for its role remains unknown. Recently, we demonstrated that Ezrin participates in EGFR/AKT signaling pathways. Importantly, transitions between active (phosphorylated open) and inactive (dephosphorylated closed) forms of Ezrin is crucial for its function in cancer. Gene expression analysis suggested dysregulation of genes that are functionally linked to cell energetic metabolism with potential implications for cell proliferation, survival and apoptosis. Total RNA was extracted from n=4 for each experimantal group: Transcriptomic analysis was performed on HeLa WT and HeLa Ezrin KO cells. Total RNA was extracted from n=4 for each experimantal group: Transcriptomic analysis was performed on HeLa WT and HeLa Ezrin KO cells.