Using BioID2 to map domain specific Ku70 protein interactors

To determine if the proximity-dependent biotin identification (BioID2) technique could identify and map domain and region specific protein interactors, we conducted BioID2 with various segments of the Ku70 protein in the HEK293 cell line and compared the different datasets. To our knowledge, this is the first study aimed at using BioID2 to directly identify domain and region specific protein interactors.