Overview of performed experiments.

The protocols that were compared and the samples that were used for each of the comparisons are listed. Yields are the average amount of DNA or RNA (μg) extracted per liter of filtered water (n/a when protocol did not extract DNA or RNA). For lysozyme treatment, the relative yield comparison in Fig. 1B was based on sample comparisons from Douglas Lake (AP optimized with and without lysozyme) and Lake Huron (Control 2 experiments). No yield data was included for the comparison of LH-APO-NT to LH-APO-RL as these derived from separate filters on which different water volumes were filtered. The abbreviations in the 16S columns correspond to those used in the figures: the first 2 letters indicate the sample site, the next 3–4 the extraction method used, and the remaining letters indicate optimizations to the protocol or differences in preservation procedure. The number(s) in parentheses indicates the sequencing run that data were generated on. N/A indicates samples were extracted for yield evaluation but not submitted for sequencing. Not included in the table are samples from Muskegon Lake that were used to compare CARD-FISH and sequencing data derived from RNAlater preserved, AllPrep optimized protocol extracted samples (ML-APO-RL (4)).Overview of performed experiments.