Transcriptional changes in bone marrow cells upon SWI/SNF inhibition [scRNA-seq]

Despite significant therapeutic potential of SWI/SNF inhibition therapy, the off-tumor effects of SWI/SNF inhibition remain largely unknown. Here, we quantify the effects of the dual SWI/SNF ATPase inhibitor BRM014 on bone marrow cells in mice. We identify lineage-dependent effects on the B and monocyte lineages within the bone marrow. Overall design: Single-cell 5' RNA-seq (scRNA-seq) of bone marrow cells from C57BL/6 mice that received 14-day treatment of daily 20 mg/kg BRM014 or vehicle control via oral gavage. All samples are pooled from 3 independent mice in the same condition and processed using 10x Chromium 5'v2 kit according to the manufacturer's instructions.