Seawater carbonate chemistry and calcification and innate immune system gene expression in juvenile American lobsters, Homarus americanus

We evaluated the effects of OA and OW on early benthic juvenile lobster transcriptomics using RNA sequencing and RT-qPCR through two distinct aquarium experiments. Lobsters were reared under OW/OA conditions aligned with values predicted for 2100: decrease in pH by 0.3–0.4 units; mean sea surface warming of 2.89 ◦C. RNA was isolated from carapace hypodermal tissue in both experiments.