Re-generation of cytotoxic ?dT cells with distinctive signatures from human ?dT-derived iPSCs

The goal of this study was to compare gene expressions on the single cell level in (i) freshly isolated PBMCs (no stimulation and no sorting), (ii) PB?dT cells; PBMCs were stimulated with HMBPP in vitro and CD3(+) ?dTCR(+) cells were sorted and (iii) i?dT cells; differentiated cells from ?dT-iPSC clone were stimulated with HMBPP and CD3(+) ?dTCR(+) cells were sorted or not sorted. Overall design: Single-cell RNA sequencing (scRNA-seq) was performed on following seven samples; S1 (freshly isolated PBMCs, Sample Tag01), S2 (CD3+gdTCR+ sorted PB-?dT cells, Sample Tag02), S3 (CD3+gdTCR+ sorted ?d Tcells differentiated from iPSCs, Exp137 d42), S4 (CD3+gdTCR+ sorted ?d Tcells differentiated from iPSCs, Exp138 d41), S5 (CD3+gdTCR+ sorted ?d Tcells differentiated from iPSCs, Exp139, d36), S6 (non-sorted ?d Tcells differentiated from iPSCs, Exp53-5A, d38) and S7 (non-sorted ?d Tcells differentiated from iPSCs, Exp54-5A, d34). Freshly isolated PBMCs, PB?dT cells and i?dT cells were captured and processed using the BD Rhapsody platform, and messenger RNA profiles of 397 genes (BD Rhapsody Immune response panel Hs) were generated by deep sequencing.