RNA secondary structome of SARS-CoV-2 and other 6 virus structures in the UTR regions

we resolved the RNA secondary structure of SARS-CoV-2 infected in Huh7.5.1 cells, in vitro structure of SARS-CoV-2 RNA purified from infected cells and refolded in test tube and viral RNA fragments of 7 coronaviruses including SARS-CoV-2, SARS-CoV and MERS-CoV based on in vivo click selective 2'-hydroxyl acylation and profiling experiment (icSHAPE). We built RNA structural model of SARS-CoV-2, found some functional structural elements, analysed the RNA structure conservation among different coronavirus. Also, we predicted RBP binding sites and identified some potential drug for CoVID-19 therapy. Overall design: RNA secondary structure of SARS-CoV-2 infected in Huh7.5.1 cells, in vitro structure of SARS-CoV-2 RNA purified from infected cells and refolded in test tube and iral RNA fragments of 7 coronaviruses including SARS-CoV-2, SARS-CoV and MERS-CoV were resolved based on icSHAPE. The RNA structure in vivo and in vitro of SARS-CoV-2 was calculated based on DMSO samples (D), in vivo NAI-N3 treatment samples (N) and in vitro NAI-N3 treatment samples (T).