Pre-separation of PBMCs using CD14-beads before application of CD1c+ DC isolation kit
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http://flowrepository.org/id/FR-FCM-ZZRT
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资源简介：
We have observed two distinct populations of cells within CD1c+ cells that are isolated using a commercially available CD1c-targeting kit: monocytes and DCs. To investigate whether we can improve the purity of CD1c+ DCs through depletion of monocytes, we applied CD14-targeting magnetic beads before applying the CD1c-kit according to the manufacturers instructions. We stained for CD45, HLA-DR, CD11c, CD14, CD11b, TREM1 and CD16
Conclusion:
Pre-depletion of monocytes by CD14-targeting beads leads to a strong enrichment of cells with clear DC phenotype in the CD1c+ preparation. Cell populations after application of "CD1c+ DC isolation kit"
创建时间：
2016-06-01
