Durability of Aligned Microtubules Dependent on Persistence Length Determines Phase Transition and Pattern Formation in Collective Motion

Collective motion is a ubiquitous phenomenon in nature. The collective motion of cytoskeleton filaments results mainly from dynamic collisions and alignments; however, the detailed mechanism of pattern formation still needs to be clarified. In particular, the influence of persistence length, which is a measure of filament flexibility, on collective motion is still unclear and lacks experimental verifications although it is likely to directly affect the orientational flexibility of filaments. Here, we investigated the collective motion of microtubules with different persistence lengths using a microtubule–kinesin motility system. We showed that local interactions between microtubules significantly vary depending on their persistence length. We demonstrated that the bundling of microtubules is enhanced by more durable alignment, rather than by greater likelihood of alignment. An agent-based computational model confirmed that the rigidity-dependent durability of microtubule alignment dominates their collective behavior.