Characterisation of epithelial and mesenchymal cells in murine pancreatic tumours

We performed RNA-seq analysis on YFP+EpCAM+ and YFP+EpCAM- cell populations freshly isolated from three Pdx1-Cre;KrasLSL-G12D;Trp53fl/fl;Rosa26LSL-YFP (KPCY) murine pancreatic tumours. We compared the transcriptome between these two populations with the aim to understand their molecular interactions that could underlie epithelial-mesenchymal plasticity and tumour heterogeneity. Overall design: Transcriptome profiles of YFP+EpCAM+ and YFP+EpCAM- cells were generated by deep RNA sequencing, in three biological replicates (primary KPCY murine pancreatic tumours).