Gene expression profiling of adipocyte and stromal vascular cells from adipose tissue in lean, obese and formerly obese mice

To investigate the transcriptional profiles of adipocytes and stromal vascular cells from mouse visceral adipose tissue(vWAT) in lean (Low fat diet, LFD fed), obese (high fat diet, HFD fed) and formerly obese (diet switch (SW) from 9 weeks HFD, to 3 weeks LFD) to induce weight loss. Overall design: Male wild type C57BL/6J mice (Jackson Laboratories) were group housed in standard cages and fed either a 10% (LFD, D12450, Research Diets, New Brunswick, NJ) or 60% fat diet (HFD, D12492) from 9-10 weeks of age. After 9 weeks of HFD feeding, half of the HFD-fed mice where switched to the LFD (SWD) for 3 weeks to induce ~10% weight loss which is sufficient to normalize glucose tolerance and insulin sensitivity to equivalent levels in observed in lean, normal chow fed mice