Anticancer activity of fungal endophytes from Datura stramonium on two human cancer cell lines

Datura stramonium is a medicinal plant of the Solanaceae family. The plant is known to produce toxic alkaloids which include atropine, hyoscyamine and scopolamine. The data sets herein represent the findings from the isolation of fungal endophytes from the stems, leaves and seeds of D. stramonium. Eleven fungal endophytes were recovered from the plant and the internal transcribed spacer region (ITS1 and ITS4) of each isolate was sequenced and the data was deposited in GenBank. Accession numbers of each fungal isolate and the phylogeny reconstruction are all shown in Supplementary File 1. Ethyl acetate crude extracts from the fungal isolates were screened for anticancer activity on two human cancer cell lines (UMG87 glioblastoma cells and A549 lung carcinoma cells) using the MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] assay. The crude extract from the fungal endophyte Alternaria sp. KTDL7 was found to be cytotoxic on UMG87 glioblastoma cells while the other fungal extracts showed limited anti-proliferation activity on both cell lines. The data cytotoxicity experiments is shown in Supplemetary File 2 and 3. Untargeted secondary metabolite screening for Alternaria sp. KTDL7 was done using LC-QTOF-MS/MS and the data is shown in Supplementary File 4.曼陀罗（Datura stramonium）为茄科（Solanaceae）药用植物，已知其可产生包括阿托品（atropine）、莨菪碱（hyoscyamine）及东莨菪碱（scopolamine）在内的有毒生物碱。本数据集涵盖从该植物的茎、叶及种子中分离真菌内生菌的相关研究结果。 本研究从该植物中共分离得到11株真菌内生菌，对每株菌株的内部转录间隔区（ITS1和ITS4）进行了测序，相关数据已提交至基因库（GenBank）。各菌株的登录号及系统发育重建结果均详见补充文件1。 采用MTS[3-(4,5-二甲基噻唑-2-基)-5-(3-羧甲基氧苯基)-2-(4-磺苯基)-2H-四唑鎓]分析法，对各菌株的乙酸乙酯粗提物开展了针对两种人类癌细胞系（UMG87胶质母细胞瘤细胞、A549肺腺癌细胞）的抗癌活性筛选。结果发现，真菌内生菌Alternaria sp. KTDL7的粗提物对UMG87胶质母细胞瘤细胞具有细胞毒性，其余菌株提取物对两种细胞系仅表现出有限的抗增殖活性。细胞毒性实验相关数据详见补充文件2与3。 采用液相色谱-四极杆飞行时间串联质谱（LC-QTOF-MS/MS）技术，对Alternaria sp. KTDL7开展了非靶向次级代谢产物筛选，相关数据详见补充文件4。