Bulk RNAseq of in vitro human Rett syndrome neurons with addition of NAD supplementation

Here we employed RNA-seq on neuronal cultures differentiated from human induced pluripotent stem cells (hIPSCs) derived from Rett syndrome patients. The study aims to understand the effects of PARP stimulation through the treatment of nicotinamide adenine dinucleotide (NAD) on gene expression of MECP2 mutant neurons. Overall design: Two isogenic hIPSC-derived patient were differentiated into neurons. R18 corresponds to the control line and R15 corresponds to the MECP2 mutant line. Mutant neurons were also treated with 10 mM of NAD for 48 hours.