scRNA-seq of human trophoblast stem cells as a model of trophoblast-cytotrophoblast (TB-CTB). scRNA-seq of human trophoblast stem cells as a model of trophoblast-cytotrophoblast (TB-CTB)

Placenta derived Okae et al. human trophoblast stem cells cultured in Okae et al. conditions (ttps://doi.org/10.1016/j.stem.2017.11.004). In addition, we sequenced SHEF6 primed human pluripotent stem cells as a control. Individual cells were manually isolated with a mouth pipette and subjected to single-cell full-lengths transcriptome profiling using a modified version of Smart-Seq2.