Table S1 - The Roles of the Catalytic and Noncatalytic Activities of Rpd3L and Rpd3S in the Regulation of Gene Transcription in Yeast

Mass spectroscopic analysis of Rpd3 isolated from cells cultured in SPM for six hours. Cells grown in SA to a density of 1 × 107 cell/ml were shifted to SPM. After six hours in SPM, proteins were crosslinked with 1% formaldehyde for 15 min. Rpd3 complexes isolated by IP were subjected to gel electrophoresis, excised from the gel, and analyzed using mass spectroscopy. Strains: MATa/MATα RPD3-13xmyc-URA3/RPD3-13xmyc-URA3 (Y1767) and MATa/MATα rpd3Δ::HIS3/rpd3 Δ::HIS3 (Y1888, control). The results for strain Y1767 are shown. Sf - score for each peptide was calculated by a neural network algorithm that incorporates the Xcorr, DeltaCn, Sp, RSp, peptide mass, charge state, and the number of matched peptides for the search. P (pep) displays the probability of finding a match as good as or better than a random match. (DOCX)