Bulk RNA-sequencing of mouse prostate tumors initiated by transplantation of engineered organoids.

Purpose: Profile global expression of prostatic tumors to compare with published datasets. Methods: Prostate tumors were flash frozen followed by processed for total RNA using the RNeasy kit (Qiagen). Samples were submitted for polyA library preparation and bulk RNA-sequencing. Conclusions: Global gene expression profiling and comparison of prostate tumor genotypes suggests differential enrichment of neuroendocrine signatures that match established human neuroendocrine prostate cancer expression signatures. Overall design: To investigate the molecular landscape of transplanted PtPM and RPM orthotopic prostate tumors, n = 10 PtPM and n = 8 RPM tumors isolated at early or late time points were subjected bulk RNA-sequencing.