Miguel Vicente-Manzanares, Karen Newell-Litwa, Alexia I. Bachir, Leanna A. Whitmore, Alan Rick Horwitz (2011) CIL:25845, Cricetulus griseus, epithelial cell. CIL. Dataset

MT inhibition alters the location of the cell front. Time-lapse of a cell expressing RLC-D,D–GFP (a phospho-mimetic mutant of the RLC) on fibronectin in the presence of 1 mM nocodazole. Note the accumulation of RLC-D,D–GFP in one region of the cell, the abnormal length and orientation of the leading edge, and the subsequent ripping of the front. mages were captured in TIRF mode using an inverted microscope (IX70; Olympus) coupled to a CCD camera (Retiga Exi; Qimaging). Live samples were acquired at 37°C using CCM1 medium. This movie corresponds to Fig. 2 A and video 3 in J Cell Biol 2011 193:381-396.

细胞前沿位置的改变与MT抑制相关。在存在1 mM 非达唑的条件下，对表达RLC-D,D–GFP（RLC的磷酸模拟突变体）的细胞在纤连蛋白上的时间序列图像进行了捕捉。请注意，RLC-D,D–GFP在细胞的一个区域积累，前端长度和方向的异常，以及随后前沿的撕裂。图像采用倒置显微镜（IX70；Olympus）配以CCD相机（Retiga Exi；Qimaging）在TIRF模式下获取。活细胞样本在37°C下使用CCM1培养基采集。此视频对应于J Cell Biol 2011 193:381-396中的图2A和视频3。