Gene expression profile at single cell level of non-parenchymal cells from Fibrosis and Tumor (FAT) Metabolic Dysfunction-Associated Steatohepatitis (MASH) model vs healthy liver at 12- and 24-week timepoints.

MASH is a subtype of metabolic dysfunction-associated steatotic liver disease, characterized by inflammation and fibrosis. Non-parenchymal cells in this dataset include mostly immune cells, as well as some other cell types like endothelial cells, cholangiocytes, stellate cells, etc. Single cell suspensions from MASH vs healthy murine livers were made using enzymatic digestion with Dnase I and Collagenase IV, as well as mechanical digestion, followed by filtering. Non-parenchymal cells were enriched by using percoll gradients (70% and 25%). Most hepatocytes are removed with this protocol, and red blood cells are lysed. Samples were submitted for scRNAseq, and were analyzed for uPAR expression in this study.