Hesperadin Sensitizes Gastric Cancer Cells to Cisplatin via NOX1-Dependent Oxidative Stress

GC cell viability was assessed via a Cell Counting Kit-8 assay. Apoptosis, intracellular reactive oxygen species levels, and the mitochondrial membrane potential were evaluated via flow cytometry and JC-1 staining. Transcriptomic analysis was subsequently conducted to identify differentially regulated pathways, followed by validation via qRT-PCR and Western blotting. DNA damage was measured by y-H2AX immunofluorescence, and clonogenic survival was examined. The NOX1-specific inhibitor ML171 was used to verify its mechanistic involvement.