Supplementary material 2-5: Agarose gel electrophoresis to assess PCR product yield: comparison with spectrophotometry, fluorometry and qPCR

Supplemental figure 1A and B: Agarose gel
after PCR.
A: Original image of agarose gel. B: Image
of agarose gel with virtually subtracted background.
Supplemental figure 2A and B: Agarose gel
after purification of PCR products.
A: Original image of agarose gel. B: Image
of agarose gel with virtually subtracted background.
Supplementary material 1: Protocol for Agarose
gel electrophoresis to assess PCR product yield – Comparison with
spectrophotometry, fluorometry and qPCR.
Supplementary table
1: PCR reaction composition
Supplementary table
2: PCR reaction parameters
Supplementary table
3: Primer sequences
Supplementary table 4: Peak area acquired
with ImageJ from agarose gel after PCR
Supplementary table 5: Peak area acquired
with ImageJ from agarose gel after PCR product purification
Supplemental material 3 containing: Supplementary
table 6: Results of spectrophotometry
Supplementary table 7: Results of
fluorometry
Supplementary table 8: Results of qPCR