Yeast communities from Nova Scotia vineyards before and after spontaneous fermentation

Vineyards were sampled in the Annapolis Valley, Nova Scotia and assessed by targeting the fungal ITS regions using both Illumina MiSeq and PacBio Sequal II systems to compare yeast communities on grapes before and after spontaneous fermentation. Bulk samples were provided from eight vineyards over three years (2018-2020) at time of harvest, with 1-2 years of sampling per vineyard. PacBio sequencing was included in one year (2019) of this sampling. Additional samples were taken in a fourth year (2021) comprising three within-vineyard replicates from seven of the same vineyards and an additional ninth, and were assessed by Illumina MiSeq. Only pre-fermentation musts were assessed of 2021 samples. The SEED2 software was used to process sequence data and the resulting, unedited, BLASTn tables are uploaded here. Curated spreadsheets filtered for yeasts with species cross-checked and names updated are also included. Datasets will be added as they are assessed. Results thus far show that pre-fermentation communities have a high diversity of basidiomycete yeasts, and some unconventional yeasts were found post-fermentation, such as Saccharomyces uvarum.本研究于加拿大新斯科舍省安纳波利斯谷的葡萄园开展采样工作，以真菌ITS区域（Internal Transcribed Spacer, ITS）为靶标，分别采用Illumina MiSeq与PacBio Sequal II测序平台，对自发发酵前后葡萄表面的酵母群落组成进行对比分析。研究团队于2018至2020年的三年间，从8家参与采样的葡萄园采集批量样品，采样时间为葡萄收获期，每家葡萄园采样1至2个年度；其中2019年的采样批次采用了PacBio测序技术。2021年，本研究额外开展第四轮采样：从原有的7家葡萄园以及新增的第9家葡萄园的同一地块内设置3个生物学重复样本，仅对2021年采集的发酵前葡萄汁（must）样本采用Illumina MiSeq平台进行测序分析。本研究使用SEED2软件对测序原始数据进行处理，未经过编辑的BLASTn比对结果表格已上传至本数据集；同时附带经过人工整理的筛选表格，该表格针对酵母类群进行了筛选，并对物种分类信息开展交叉验证与名称更新。本数据集将随后续分析进度持续更新。目前已获得的初步分析结果显示，发酵前葡萄汁的微生物群落具有较高的担子菌酵母多样性；且在发酵后样本中发现了部分非常规酵母类群，如葡萄汁酵母（Saccharomyces uvarum）。