Interactome of DYRK2 by using BioID proximity labeling

In this study, DYRK2 was found to be an important regulator of Hedgehog signaling. To identify DYRK2 interacting proteins, we performed interactome using BioID proximity labeling. NIH3T3 cells containing Dox-inducible DYRK2-BioID2-HA construct were cultured in the media with or without Doxycycline for DYRK2-BioID2-HA expression, followed by proximity labeling. The lysates from Doxycycline induction (DYRK2) and without induction (Mock) were subjected to streptavidin pull-down and the proteins were identified by LC-MS/MS.