Electrophoretic mobility shift assay to assess nuclear factor (NF)-?B (, Supershift) and p53 () DNA binding activity

Nuclear extracts of hepatic stellate cells (HSCs) at days 2 and 7 in control and in atorvastatin 10 mol/L were assessed using a NF-κB and p53 binding site as probe. Please note that in both quiescent and activated HSC, there are no differences between the control and the cells treated with atorvastatin. We could confirm the data in five different blots of five independent experiments.<b>Copyright information:</b>Taken from "Atorvastatin induces apoptosis by a caspase-9-dependent pathway: an study on activated rat hepatic stellate cells"Liver International 2008;28(4):546-557.Published online 01 Apr 2008PMCID:PMC2324535.Liver International (2008) © The Authors. Journal compilation © 2008 Blackwell Munksgaard