RNA-seq of blood and adipose tissue from mice infected with Trypanosoma brucei. RNA-seq of blood and adipose tissue from mice infected with Trypanosoma brucei

We asked whether the in-vivo transcriptome of T. brucei AnTat 1.1E 90-13 parasites collected from adipose tissue and blood is different. We collected gonadal adipose tissue of infected mice on day 6 post-infection, when a high number of parasites is expected, and blood on day 4 post-infection when slender-forms are the most abundant form of the parasite. Parasites from blood were purified over a DEAE column. Total RNA was extracted from two independent samples of blood and three independent samples of fat and subjected to RNA-sequencing.