Polysome loading state of selected mRNAs in eif3h mutant versus wild type.

Note: TL eif3h/WT. Translation state (TL) is the ratio of mRNA in polysomal and non-polysomal RNA fractions; it is log-transformed and unitless. When compared between eif3h mutant and wild-type seedlings, negative numbers indicate that the mRNA is less polysomal, i.e. undertranslated in the eif3h mutant. The values are means from duplicate polysome microarrays [13]. TOADSTOOL and the heterodimer of CLV2 and CORYNE are receptor like kinases that can function as CLV3 receptors in parallel to CLV1. Data for ARFs, TIR1 and AUX1 are included for the purpose of calibration, given that ARFs are also undertranslated in eif3h while TIR1 and AUX1 are not [16]. The median value from 8832 genes was −0.10±0.21. TOADSTOOL and AS1 and CLV1 rank 19th and 55th and 495th, respectively, among cytosolic mRNAs. uORF numbers are from TAIR10. NA, not available.