Myxococcus xanthus Raw sequence reads

We characterized a new S-motility gene in Myxococcus xanthus. This mutant is temperature-sensitive (Ts) for S-motility; however, Tfp and EPS are made. This mutation was in strain DK1649. To identify the mutation, we sequenced the genome of DK1649 and compared it to the wild-type (WT) DK1622 reference genome. This analysis revealed many mutations that confounded gene identification. Fortuitously, however, we isolated three revertants of DK1649 that restored S-motility. These isolates were sequenced and were all found to contain a single base insertion in MXAN_4099, compared to the DK1649 genome, which restored the WT DK1622 sequence. A 1 bp deletion was mapped to the MXAN_4099 locus and the gene was named sglS.