Quantitative analysis of the DNA methylation sensitivity of transcription factor complexes
收藏NIAID Data Ecosystem2026-04-25 收录
下载链接：
https://www.ncbi.nlm.nih.gov/sra/SRP106628
下载链接
链接失效反馈官方服务：
资源简介：
EpiSELEX-seq method was used to characterize the binding preference of human TF complexes to methylated DNA, including BZIP proteins ATF4 and CEBPb, homeodomain TF heterodimers of HM(Meis1)-Pbx1 together with either HoxA1, HoxA5 or HoxA9 and tumor suppressor protein p53. Overall design: One round of EpiSELEX-seq was performed for all TFs tested as described in Kribelbauer et al. Purified TFs were incubated with a mix of methylated and unmethylated randomized 16bp (26bp for p53) DNA libraries of sequence GGTAGTGGAGG-TGGG-CCTGG-16xN/26xN-CCAGG-GAGGTGGAGTAGG for unmethylated and GGTAGTGGAGG-GCAC-CCTGG-16xN/26xN-CCAGG-GAGGTGGAGTAGG for methylated library. Bound fractions were amplified and sequenced.
创建时间：
2019-09-23
