Single-cell transcriptome analysis of tumor infiltrated CD45+ immune cells isolated from ctrl- or thonzonium bromide- treated mice

As a monocationic detergent, thonzonium bromide (TB) has been approved as an additive to pharmacologic formulations, serving to promote tissue contact of the administered drugs by dispersion and penetration of the cellular debris and exudate, but has not been used for cancer treatment. In this study, we performed single-cell RNA sequencing (scRNA-Seq) to identify the composition of the immune microenvironment after TB treatment. Overall design: Tumor infiltrated CD45+ immune cells were isolated by Fluorescence-activated cell sorting (FACS) from PBS or thonzonium bromide treated tumor-bearing mice.