Whole-exome sequencing analysis of HNM007, AKR, and NOOC1 cells.

To characterize the mutational status of mouse head and neck squamous cell carcinoma (HNSCC) cell lines, we performed whole-genome sequencing analysis on HNM007, AKR, and NOOC1 cells. Genomic DNA was extracted from HNM007, AKR, and NOOC1 cells by phenol–chloroform extraction and then purified with TIANamp Genomic DNA Kit (Spin Column). Whole-exome libraries were constructed with the Agilent SureSelectXT Mouse All Exon Kit. Libraries were constructed using 600 ng of DNA and were sequenced on the Illumina NovaSeq 6000 platform using 150 bp paired-end reads at the Rhelixa Inc, Tokyo, Japan.