Isolation and characterization of cDNA clones encoding a functional p34cdc2 homologue from Zea mays.
收藏PubMed Central1991-04-15 更新2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC51450/
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资源简介：
We describe the isolation of cDNA clones encoding a p34cdc2 homologue from a higher plant, Zea mays (maize). A full-length cDNA clone, cdc2ZmA, was isolated, sequenced, and shown to complement a cdc28 mutation in Saccharomyces cerevisiae. Comparison of the deduced amino acid sequence of the maize p34cdc2 protein with other homologues showed that it was 64% identical to human p34cdc2 and 63% identical to Schizosaccharomyces pombe and S. cerevisiae p34cdc2 proteins. Studies of expression of the maize cdc2 gene(s) by Northern blot analysis indicated a correlation between the abundance of cdc2 mRNA and the proliferative state of the tissue. Southern blot analysis, as well as isolation of another cDNA clone, cdc2ZmB, which is 96% identical to cdc2ZmA, indicates that maize has multiple cdc2 genes. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1991-04-15
