SURVEILLANCE AND MOLECULAR EPIDEMIOLOGY OF ESPECIALLY DANGEROUS RESPIRATORY VIRUSES IN COMMERCIAL AND BACKYARD POULTRY AND MIGRATORY WATERFOWL IN INDIA

Samples were collected from various avian species across Kerala, Haryana and Odisha, India between 2018-2020. All samples were tested for the presence of NDV (Newcastle disease virus) using RT-PCR based on the matrix gene (M) of the virus. Samples positive for the M gene were further tested with F gene primers to determine the pathotype of NDV. In addition, pools from all samples (five samples to a pool) from the three centers were tested for avian influenza virus (AIV) followed by testing of individual samples from positive pools.本数据集的样本采集自印度喀拉拉邦、哈里亚纳邦与奥里萨邦的多种禽类，采集时间为2018年至2020年。所有样本均基于病毒的基质基因（M基因），采用逆转录聚合酶链式反应（RT-PCR）检测新城疫病毒（NDV, Newcastle disease virus）的携带情况。基质基因检测呈阳性的样本，将进一步使用F基因引物开展检测，以明确新城疫病毒的致病型。此外，来自三个采样中心的全部样本均按每5个一组构建为混合池，所有混合池先针对禽流感病毒（AIV, avian influenza virus）进行检测，随后对阳性混合池中的单个样本开展复核检测。