The effect of beta-glucan - induced trained immunity on the epigenetic profile of bone marrow granulocyte-macrophage progenitors (GMP) and neutrophils

Induction of trained immunity by beta-glucan affects myeloid cells and their bone marrow progenitors. In particular, broad epigenetic alterations in trained myeloid cells have been demonstrated. In this study, we performed single cell ATAC sequencing in GMP and neutrophils from beta-glucan treated mice, as compared to control-treated mice, in order to investigate the impact of trained immunity on the epigenetic profile of GMP and neutrophils. WT mice were treated with a single dose of 1 mg of β-glucan from Trametes versicolor (Invivogen) in PBS or with PBS vehicle alone (control) and after seven days bone marrow GMP (Lin—c-Kit+Sca1—CD16/32+CD34+) and splenic neutrophils (CD11b+Ly6c-Ly6g+) were sorted and ATAC sequencing analysis was performed.