Hybrid dysgenesis of natural strains in Drosophila and piRNA. Drosophila melanogaster

This project is to reveal the production of piRNAs associated with hybrid dysgenesis of natural strains in Drosophila melanogaster. The RNA-seq was performed using 20 hybrids having various ability to resist P transposable elements. Various levels of small RNA were obtained from their ovaries and embryos. These data support the different production of piRNAs from P transposable elements between natural strains. The amplicon-seq was performed using 4 lines of natural strains in Drosophila melanogaster. These PCR amplicon were contained vicinity sequence of P transposable elements to detect insertion site of P transposable elements. These data support insertion site of P transposable elements, including piRNA clusters which has activity to produce piRNAs