Exploring the impact of biotic and abiotic surfaces on protein binding

The oral environment is composed of a diverse array of proteins, and any substrate inserted into this habitat promptly becomes subjected to protein adsorption and bacterial colonization. However, the predictive and modulatory nature of the surfaces coated with salivary pellicle proteomes in microbial adhesion has not been explored. Thus, using human saliva for salivary pellicle adsorption and for biofilm formation, we compared adsorption and community formation on titanium (Ti) biomaterial (implant devices) and dental surfaces (enamel and dentine). The proteomic profile was evaluated by Liquid chromatography coupled with tandem mass spectrometry and microbiome by 16S RNA sequencing.