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Additional file 2 of Effect of phosphorus supply on root traits of two Brassica oleracea L. genotypes

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Additional file 2 Table S1. Two-way analysis of variance (ANOVA) table with p values (those less than 5% are highlighted in bold) and means squares (in italics) for response variables in two Brassica oleracea accessions (C6 and F103) grown hydroponically for 2 weeks in solutions containing 0.025 mM P (Low P) or 0.25 mM P (High P); Zn, zinc. Table S2. Relative concentration of polar metabolites detected in root exudates of two Brassica oleracea accessions (C6 and F103) grown hydroponically for 2 weeks in solutions containing 0.025 mM P (Low P) or 0.25 mM P (High P). * indicates metabolites for which fold change could not be calculated, as it was not detected in one of the treatments in both accessions. Table S3. Two-way analysis of variance (ANOVA) table with p values (those less than 5% are highlighted in bold) and means squares (in italics) for main root length and ANOVA on Ranks for lateral root length along the main root length, divided into quartiles, in two Brassica oleracea accessions (C6 and F103) grown in the high throughput root phenotyping system with Low phosphorus (P) and High P treatments (four groups in total) for 2 weeks. Table S4. All differentially expressed P-responsive genes and their expression in FPKM, in roots of two Brassica oleracea accessions (C6 and F103) grown hydroponically for 2 weeks in solutions containing 0.025 mM P (Low P; LP) and 0.25 mM P (High P; HP). Differentially expressed genes were defined by comparing the gene expression in the Low P treatment with the gene expression in the High P treatment for each of the B. oleracea accession. Table S5. Gene ontology enrichment analysis for all differentially expressed genes in roots of two Brassica oleracea accessions (the C6 and the F103) grown in solutions containing 0.025 mM P (Low P) and 0.25 mM P (High P) for 2 weeks (n = 3 for each accession and each treatment). Bonferroni corrected p-values were used to determine significantly enriched categories and a corrected p-value of 0.05 was used as a cut-off. Table S6. P-responsive genes with orthologues in Arabidopsis thaliana. The table shows the gene expression of all P-responsive genes in the roots of two Brassica oleracea accessions (C6 and F103) grown hydroponically for 2 weeks in solutions containing 0.025 mM P (Low P) and 0.25 mM P (High P) which have A. thaliana ortholouges. Table S7. Shoot fresh weight, phosphorus (P) and zinc (Zn) concentrations and P efficiency ratio (calculated as Yieldlow / (Plow × Yieldlow) or Yieldhigh / (Phigh × Yieldhigh)) of the two Brassica oleracea accessions (C6 and F103) grown in a peat-based compost amended with Low P (5.25 mg L− 1) and High P (15.75 mg L− 1) used in the experiments. Data for fresh weight, shoot P concentration and P efficiency ratio are from [1] and shoot Zn concentrations are from [2]; DM, dry matter.
提供机构:
Hiram Castillo-Michel; Matevž Likar; Primož Vavpetič; Primož Pelicon
创建时间:
2020-08-06
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