Array comparative genomic hybridization of Canine Oral Melanoma (COM) archival samples.. Array comparative genomic hybridization of Canine Oral Melanoma (COM) archival samples.

The aim of this work was to identify the Copy Number Aberrations (CNAs) by high-resolution array Comparative Genomic Hybridization (aCGH) on 19 formalin-fixed, paraffin-embedded (FFPE) samples of treatment-naïve COM. Overall design: In total, the DNA obtained from 19 COM specimens together with the paired healty tissue present in the same FFPE block were analyzed using a Agilent-025522 SurePrint G3 Canine CGH Microarray 4x180K (GPL10156). Tumor and reference DNA samples were labelled independently with cyanine 5-deoxyuridine triphosphate (dUTP) and cyanine 3-dUTP, respectively, using SureTag Complete DNA Labeling Kit. Oligo aCGH analysis was performed following the manufacturer’s recommendations, using an Oligo aCGH/ChIP-on-chip Hybridization Kit (Agilent Technologies).