RNAseq of partially paralyzed zebrafish embryos at 5 days post-fertilization compared to normal siblings

Sofa potato (sop) is a mutant zebrafish line, whose synaptic transmission at the neuromuscular junction is absent due to a point mutation in the δ subunit gene of the acetylcholine receptor (AChR), leading to paralysis of its skeletal muscles. To explore genetic changes in embryos caused by the lack of synaptic transmission, we performed RNA-seq analysis of normal siblings (sop+/?) and sop homozygous embryos (sop-/-) at 5 days post-fertilization., Sofa potato (sop) is a mutant zebrafish line, whose synaptic transmission at the neuromuscular junction is absent due to a point mutation in the δ subunit gene of the acetylcholine receptor (AChR), leading to paralysis of its skeletal muscles (doi:10.1523/jneurosci.0851-04.2004). To explore genetic changes in synapses caused by the lack of synaptic transmission, we performed RNA-seq analysis of normal siblings (sop+/?) and sop homozygous embryos (sop-/-) at 5 dpf. Sop-/- had an inducible wild type δ subunit gene, which was induced at 2 dpf resulting in partial paralysis (doi:10.1038/s41598-017-01900-3). Zebrafish embryos obtained from a cross of sop+/- were separated into the paralyzed homozygous group (sop-/-) and the swimming siblings (sop+/+ or sop+/-). Both groups were subjected to mifepristone to induce wild type delta subunit to partially rescue paralyzed fish. Total RNA was extracted from both groups (~10 larvae in each) at 5 dpf using RNeasy Mini Kit (QIAGEN). Next-Generation se..., , # RNAseq of partially paralyzed zebrafish embryos at 5 days post-fertilization compared to normal siblings

[https://doi.org/10.5061/dryad.7d7wm3838](https://doi.org/10.5061/dryad.7d7wm3838)

RNAseq data derived from zebrafish embryos at 5 days post fertilization, to compare the effect of paralysis.

## Description of the data and file structure

Sofa potato (sop) is a mutant zebrafish line, whose synaptic transmission at the neuromuscular junction is absent due to a point mutation in the δ subunit gene of the acetylcholine receptor (AChR), leading to paralysis of its skeletal muscles (doi:10.1523/jneurosci.0851-04.2004). To explore genetic changes in synapses caused by the lack of synaptic transmission, we performed RNA-seq analysis of normal siblings (*sop*+/?) and sop *homozygous embryos* (sop-/-)  at 5 dpf. Sop-/-  had an inducible wild type δ subunit gene, which was induced at 2 dpf resulting in partial paralysis (doi:10.1038/s41598-017-01900-3). Zebrafish embryos obtained from a c...