Plasma Proteomics Analysis of the Cooperative Health Research in South Tyrol (CHRIS) Study, QC Samples

The Cooperative Health Research in South Tyrol (CHRIS) study is a single-site population-based study aimed to investigate the genetic and molecular basis of common age-related chronic conditions and their interaction with lifestyle and environment. In recent work, we have been evaluating the impact of age, sex and diet, amongst others, on the human metabolome. Moreover, gene-metabolite associations, as well as genetic and metabolomic determinants of disease, have been investigated. Using Scanning SWATH acquisition on Triple TOF 6600 instruments (Sciex) we created a MS-based plasma proteomics data set with low technical variability for n = 3,632 CHRIS participants. We performed a general exploratory analysis of the data set to identify relevant factors affecting the plasma proteome, including commonly used drugs. We found hormonal contraceptives to be the main factor explaining the variation in this data set. Here we present the commerical plasma samples used as quality controls.