Cytotoxicity and antiviral activity of 3-α-tigloyl-melianol and melianone against DENV-2 and WNV.

Data represent mean values of three independent determinations. The variation among them was less than 15% respect to the mean value.a Compound concentration (μM) required to reduce the proliferation of mock-infected BHK-21 cells by 50%, as determined by the MTT method.b-c Compound concentration (μM) required to achieve 50% protection of BHK-21 cells from respectively DENV-2(b) and WNV(c) induced cytopathogenicity, as determined by the MTT method.d-e Compound concentration (μM) required to reduce the plaque number of WNV by 50% in BHK-21 monolayers, adding compounds during infection(d) or 2 hours post infection(e).Cytotoxicity and antiviral activity of 3-α-tigloyl-melianol and melianone against DENV-2 and WNV.