Transcriptional response of Candida albicans to the utilization of amino acids as the sole carbon source

Purpose of this study was to define the transcriptional response of Candida albicans to the utilization of amino acids as the sole carbon source (C-source) in order to study regulation of metabolic adaptation of the fungus to amino acid-rich media. Furthermore, potential regulatory activities of the transcription factor Stp2 exceeding the described role in amino acid-uptake have been investigated. Therefore, the SC5314 wild type and a deletion strain of Stp2 (stp2?) were incubated in medium containing either glucose (SD) or a mixture of proteinogenioc amino acids (SC) as the sole C-source and the cellular transcriptome defined using RNA-sequencing. Overall design: Indicated C. albicans strains were pre-incubated in SD minimal medium and transferred to experimental medium containing only the indicated C-source (SD, SC) and incubated for 1 h. Cellular transcriptomes were assessed via RNA-Sequencing in biological triplicates.