Effect of TP421 treatment on DNA damage and apoptosis induction.

(A) BxPC-3 and MIA PaCa-2 cells treated with increasing concentrations of TP421 for 12–48 h were probed for the DNA damage marker phospho-H2A.X (S139). (B) MIA PaCa-2 cells treated with TP421 were probed for caspase activation and PARP-1 cleavage. (C) The effect of TP421 treatment on Bcl-2 and survivin levels was analyzed in MIA PaCa-2 cells.