Assessment of four in vitro phenotypic biofilm detection methods in relation to antimicrobial resistance in aerobic clinical bacterial isolates

Introduction: The lack of standardized methods for detecting biofilms continues to pose a challenge to microbiological diagnostics since biofilm-mediated infections induce persistent and recurrent infections in humans that often defy treatment with common antibiotics. This study aimed to evaluate diagnostic parameters of four in vitro phenotypic biofilm detection assays in relation to antimicrobial resistance in aerobic clinical bacterial isolates.
Methods: In this cross-sectional study, bacterial strains from clinical samples were isolated and identified following the standard microbiological guidelines. The antibiotic resistance profile was assessed through the Kirby-Bauer disc diffusion method. Biofilm formation was detected by gold standard tissue culture plate method (TCPM), tube method (TM), Congo red agar (CRA), and modified Congo red agar (MCRA). Statistical analyses were performed using SPSS version 17.0, with a significant association considered at p<0.05.
Result: Among the..., The relevant sample details (type, fulfillment of sample collection for microbiological standard) and their associated laboratory findings (bacteria, antimicrobial susceptibility testing, biofilm formers or non-biofilm formers) were initially recorded in the patient information sheet, and later entered in Microsoft Excel version 10.0. Any missing or ambiguous records were gathered and clarified through communication with involved healthcare workers., Microsoft Excel veriosn 10.0. would be required to open the data file with this submission.