Screening of Rhizosphere Nitrogen Fixing, Phosphorus and Potassium Solubilizing Bacteria of Malus sieversii (Ldb.) Roem.

Eight populations of organic phosphorus solubilizing bacteria (oPSB) (56), inorganic phosphorus solubilizing bacteria (iPSB) (13), NFB (22), and KSB (24) were isolated from eight populations of Xinjiang wild apple rhizosphere in Ili by medium culture. Their morphological characteristics were recorded and their activity was determined.The L16 (45) orthogonal experiment, which used the three strains as testing materials, was created to assess the effects of the strains on apple physiological indicators, soil nutrients, leaf nutrients, and biomass, as well as to identify the ideal combination, concentration, timing, and application method.研究人员采用培养基培养法，从伊犁地区8个新疆野苹果根际生境中，分离得到解有机磷细菌（organic phosphorus solubilizing bacteria，oPSB）56株、解无机磷细菌（inorganic phosphorus solubilizing bacteria，iPSB）13株、固氮菌（NFB）22株以及钾细菌（KSB）24株，其中解有机磷细菌分属8个种群。研究人员记录了所有菌株的形态特征并测定其活性。为评估上述菌株对苹果生理指标、土壤养分、叶片养分及生物量的影响，并筛选最优组合、施用浓度、施用时期与施用方式，本研究以三类菌株为试验材料，构建了L16(4^5)正交试验。